Transcriptomic technologies have revolutionized the study of gene expression and RNA biology. Different RNA sequencing methods enable the analyses of diverse species of transcripts, including their abundance, processing, stability, and other specific features. Mitochondrial transcriptomics has benefited from these technologies that have revealed the surprising complexity of its RNAs. Here we describe a method based upon cyclization of mitochondrial RNAs and next generation sequencing to analyze the steady-state levels and sizes of mitochondrial RNAs, their degradation products, as well as their processing intermediates by capturing both 5' and 3' ends of transcripts. ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2021
• January 2021

Early life is marked by distinct and rapidly evolving immunity and increased susceptibility to infection. The vulnerability of the newborn reflects development of a complex immune system in the face of rapidly changing demands during the transition to extra-uterine life. ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2021
• September 2021

This study investigated where preschool children were more or less active in outdoor play areas in Early Childhood Education and Care (ECEC) centers using a novel method of combined device-measured physical activity, spatial data and on-site audit data. ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2022
• August 2022

Tim Jones TW MBBS DCH FRACP MD Co-head, Diabetes and Obesity Research Co-head, Diabetes and Obesity Research Areas of research expertise: Diabetes Mellitus, youth, hypoglycaemia, metabolism As well as Head of Diabetes and Obesity Research at ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2017
• August 2017

Our goal was to identify genetic risk factors for severe otitis media (OM) in Aboriginal Australians. ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2021
• November 2021

The recent increase in babies born with brain and eye malformations in Brazil is associated with Zika virus (ZIKV) infection in utero. ZIKV alters host DNA methylation in vitro. Using genome-wide DNA methylation profiling we compared 18 babies born with congenital ZIKV microcephaly with 20 controls. We found ZIKV-associated alteration of host methylation patterns, notably at RABGAP1L which is important in brain development, at viral host immunity genes MX1 and ISG15, and in an epigenetic module containing the causal microcephaly gene MCPH1. Our data support the hypothesis that clinical signs of congenital ZIKV are associated with changes in DNA methylation. ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2021
• February 2021

Charlie Rebecca Christopher McLeod Pavlos Blyth CM RP CC MBBS (Hons), FRACP, PhD, DTM&H, DCH PhD, MSFS, MBBS (Hons) DCH FRACP FRCPA PhD Paediatric Infectious Diseases Clinician Researcher, Research Fellow, Raine Fellow, Deputy Director of the ...

• 1. Telethon Kids Institute
• Projects

Our results show perturbation of host mitochondrial function following T. gondii infection that likely impacts on pathogenesis of disease. ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2017
• December 2017

IL8RA and IL8RB, encoded by CXCR1 and CXCR2, are receptors for interleukin (IL)-8 and other CXC chemokines involved in chemotaxis and activation of... ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2011
• December 2011

Otitis media (OM) is among the most common illnesses of early childhood, characterised by the presence of inflammation in the middle ear cavity... ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2011
• February 2011

Maternal pre-pregnancy obesity has been linked with an increased risk for negative emotionality and inattentiveness in offspring in early childhood. ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2013
• January 2013

The absence of a diagnostic test for acute rheumatic fever (ARF) is a major impediment in managing this serious childhood condition. ARF is an autoimmune condition triggered by infection with group A Streptococcus. ...

• 1. Telethon Kids Institute
• Our research
• Reports and Findings
• 2021
• September 2021